[ effects of hearing of holy Quran voice on immune system factors in human]

Background: The aim of this study was to investigate the effects of two types of quran voice, Majlisi or Tartil on IgA, IgM, IgG, C3, C4 and White Blood Cell count.

Methods and Materials: this study was conducted on 60 volunteer students in sabzevar university of Medical sciences. Subjects were divided randomly into 2 groups. In the first group, participants heard to Majlisi voice and in the second group, subjects heard to Tartil voice of Quran for 20 minutes. Blood sampling were done before and after Quran hearing. IgA, IgM, IgG, C3 and C4 were measured by SRID and kit method and white blood cell count was done by cell counter. Data were analyzed using SPSS16 software and U Mann – Whitney and Kruskal Wallis tests.

Results: Hearing the Majlisi voice significantly increased the IgG [p=0.032], C3 [p=0.049] and C4 [p=0.004], but it did not change other factors. Hearing the Tartil voice rose significantly gust the IgG [p=0.008] and C3 [p=0.001].

Conclusion: The results of this study shows that hearing to both types of Quran voice [Majlisi or Tartil] can go up the immune system factors and inhance this to encountering some stressful and critical situations.

[Investigating Puberty Onset and Its Relationship with Body Mass Index in Boys from Sabzevar, Iran]

Background and Purpose: Evaluation of puberty stages is important to assess adolescent health. This study has been done with the aim of determination of the average age of onset of puberty and its relationship with Body Mass Index [BMI] in male students of Sabzevar city in the North East of Iran in 1390.

Methods and Materials: This cross - sectional study was conducted on 252 male students of secondary schools and high schools of Sabzevar, Iran, with the age of 11-18 years who were selected by probability and multistage sampling. Data were collected through a Tanner standardized questionnaire; afterwards, measuring of height and weight and BMI calculation were performed. Data were analyzed in SPSS 15 using chi-square test, t-test, ANOVA and Bonfronie post hoc test, analysis of covariance, Spearman correlation coefficient and Kappa agreement coefficient; significance level was considered as 0.05.

Results: To conduct this study, we selected a group of researchers who were trained for the initiation of project for assessing puberty in male schools in order to increase the accuracy of determining the Tanner stages. Students were examined for their height and weight, size of penis and testicles, and the manner of pubic and axillary hair growth as well as also voice changes. Mean final growth stage of testicles and scrotum [G5 stage], pubic hair [P5 stage], were measured to be 16.40 +/- 1.65 and 16.79 +/- 1.19 years respectively, and their mean BMI at P2 and G2 were 20.96 +/- 4.1 and 19.49 +/- 3.66 kg /m2 respectively.

Conclusion: Regarding to the declining age of puberty and its relationship with BMI, health systems should focus on training programs and encouraging teenagers to begin changing their lifestyle. Age of puberty onset in male students is similar to many other countries but determining the precise status of puberty in Iranian children requires further studies in different provinces, rural and urban areas as well as longitudinal studies.

Characterization and Prognostic Value of Mutations in Exons 5 and 6 of the p53 Gene in Patients with Colorectal Cancers in Central Iran

BACKGROUND/AIMS: We aimed to investigate the relation-ships among various mutations of the p53 gene and their protein products, histological characteristics, and disease prognosis of primary colorectal cancer in Isfahan, central Iran. METHODS: Sixty-one patients with colorectal adenocarcinoma were enrolled in the study. Mutations of the p53 gene were detected by single-stranded conformation polymorphism and DNA sequencing. The protein stability was evaluated by immunohistochemistry. Patients were followed up to 48 months. RESULTS: Twenty-one point mutations in exons 5 and 6 were detected in the tumor specimens of 14 patients (23%). Of those, 81% and 9.5% were missense and nonsense mutations, respectively. There were also two novel mutations in the intronic region between exons 5 and 6. In 11 mutated specimens, protein stability and protein accumulation were identified. There was a relationship between the type of mutation and protein accumulation in exons 5 and 6 of the p53 gene. The presence of the mutation was associated with an advanced stage of cancer (trend, p<0.009). Patients with mutated p53 genes had significantly lower survival rates than those with wild type p53 genes (p<0.01). CONCLUSIONS: Mutations in exons 5 and 6 of the p53 gene are common genetic alterations in colorectal adenocarcinoma in central Iran and are associated with a poor prognosis of the disease.

Study of relationship between p53 protein stability and pathological parameters in colorectal cancer by immunohistochemistry method

Genetic damages and dietary habits play important parts in colorectal cancer [CRC]. p53 protein, a product of p53 gene, is the most important tumor suppressor. The rate of p53 mutation and expression has been variously reported across anatomical regions. p53 protein has a short half-life which tends to increase with mutation and is likely to be traced by immunohistochemistry. This study is intended to determine the p53 protein stability by pathological parameters across different areas in CRC. This descriptive analytical research was conducted on 80 CRC cases admitted to Hospital in Isfahan, Iran from 2003 to 2007. p53 expression was detected by immunohistochemical methods in the samples after fixation, tissue processing and antigen retrieval. The obtained data were analyzed using chi-square. of the 80 specimens investigated, p53 protein stability was observed in 27 specimens [34%]. No significant relationships were observed between p53 protein stability and tumor staging, differentiation and anatomical regions [colon and rectum] but the relationship between protein stability and mutation was significant. p53 protein stability was observed in many mutated samples. Therefore, p53 protein detection in Cancer cases can be considered an important symptom of mutation signifying the prognosis and progress of cancer

Seroepidemiology of anti-rubella virus in pre-marriage girls of sabzevar, Iran by ELISA method

Background and Purpose: Rubella is a member of togaviridate viruses, with minor morbidity in children. Rubella infection during pregnancy causes various malformations including congenital cataract. Immunity against it is, therefore, important in girls before marriage and it is recommended in girls without antirubella antibody in some countries. This study is intended to determine the anti-rubella immunity in premarriage girls in Sabzevar, Iran

Methods and Materials: This descriptive-analytical study was conducted on 186 pre-marriage girls in Sabzevar, Iran to determine serological anti-rubella [IgG and IgM] using ELISA. NOVUM DIAGNOSTICA Kit was used for tests and SPSS for data analysis

Results: 172 girls [92.5%] were positive and 14 girls [7.5%] negative, i.e. high risk group.152 girls [81.7%] already infected with rubella were now positive and 11 girls [5.9%] negative

Conclusion: Subjects' age and IgG antibody did not show any significant relationships while a significant relationship was found between exposure to rubella and IgG [p<0.0019]; in other words, 81.7% of the IgGpositive cases were already exposed to rubella and 5.9% of the cases with history of rubella were IgG negative. Therefore, the high risk group is recommended to be vaccinated prior to pregnancy to prevent the risk of congenital rubella syndrome